乌珠穆沁羊不同组织基因组 DNA 甲基化状态的MSAP 分析

2017-07-19 00:17:18

何小龙1,付绍印1,李蓓2,祁云霞1,王标1,特日格勒1,郝晨芳1,达赖1,刘永斌1*,荣威恒1

(1.内蒙古自治区农牧业科学院,内蒙古呼和浩特 010031;2.内蒙古农业大学动物科学学院,内蒙古呼和浩特 010018)

摘 要:本研究应用甲基敏感扩增片段多态性方法(MSAP),选用10对选择性引物分别对5只乌珠穆沁羊的心脏、肝脏、脾脏、肺脏、肾脏、胃和背最长肌组织样品混池DNA中CCGG位点进行了甲基化检测。通过聚丙烯酰胺凝胶电泳检测共获得条带1 249条,其中TypeⅡ型条带154条,TypeⅢ型条带231条;通过计算不同组织的甲基化率,乌珠穆沁羊的心脏、肝脏、脾脏、肺脏、肾脏、胃和背最长肌平均甲基化率为37.58%、39.88%、50.63%、25.82%、20.53%、28.19%、19.21%;在所检测组织中,脾脏组织的甲基化程度最高,肌肉组织的甲基化程度最低。本研究为进一步了解甲基化在绵羊组织分化过程中所起到的作用奠定了基础。

关键词:乌珠穆沁羊;组织;甲基化;MSAP

中图分类号:S826.2

文献标识码:A

  DOI编号:10.19556/j.0258-7033.2017-07-035

Analysis of DNA Methylation in Different Ujumqin Sheep Tissues with MSAP

HE Xiao-long1, FU Shao-yin1, LI Bei2, QI Yun-xia1, WANG Biao1, TE Ri-Ge-Le1, HAO Chen-fang1, DA Lai1, LIU Yong-Bin1*, RONG Wei-heng1

(1. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Inner Mongolia Hohhot 010031, China; 2. College of Animal Science, Inner Mongolia Agricultural University, Inner Mongolia Hohhot 010018, China)

Abstract: The CCGG sites in genomes were detected among heart, liver, spleen, lung, kidney, stomach and longissimus muscle of 5 Ujumqin sheep for 10 pairs of selective primers by methylation sensitive amplified polymorphism (MSAP) method. A total of 1249 DNA fragments were detected by the polyacrylamide gel electrophoresis, including 154 type Ⅱ and 231 type Ⅲ fragments. Through calculation the methylation rates of different tissues for Ujumqin sheep, the average methylation rate of heart, liver, spleen, lung, kidney, stomach and longissimus muscle was 37.58%, 39.88%, 50.63%, 25.82%, 20.53%, 28.19%, 19.21% respectively. For the all detected tissues, the spleen tissue methylation degree was The highest and the muscle tissue was the lowest. This study laid the foundation for further understanding of the role of DNA methylation regulatory effect for the sheep tissue differentiation.

Key words:Ujumqin Sheep; Tissue; Methylation; MSAP

《中国畜牧杂志》2017年7月第7期 目录

上一篇回2017年7月第7期目录 下一篇 (方向键翻页,回车键返回目录)加入书签

© 2016 毕业论文网 > 乌珠穆沁羊不同组织基因组 DNA 甲基化状态的MSAP 分析