黑萨福克和杜泊羊新鲜和冷冻胚胎移植效率的研究

2017-08-04 23:13:28

徐志远1,2,杨明辉2,姬鹏云2,张效生3,张金龙3,朱士恩2,阿布力孜·吾斯曼4,赵静1*,刘国世2*

(1.吉林农业大学动物科技学院,吉林长春 130118;2.中国农业大学动物科技学院,北京 100193;3.天津市畜牧兽医研究所,天津 300381;4.新疆农业大学动物科学学院,新疆乌鲁木齐 830052)

摘 要:为提高优质肉用种用绵羊胚胎移植的效率,试验选取76只黑萨福克羊和27只杜泊羊作为供体,采用“CIDR+FSH+PMSG ”法进行超数排卵处理。选取384只小尾寒羊为受体,采用“CIDR+PMSG”法进行同期发情处理,以探索供体胚胎发育阶段、胚胎冷冻处理、胚胎体外停留时间以及移植给受体时移植侧黄体数量等因素对胚胎移植妊娠率的影响。 结果表明:移植新鲜囊胚的妊娠率极显著高于桑椹胚(P<0.01);冷冻/解冻囊胚移植妊娠率极显著低于新鲜囊胚(P<0.01),而冷冻/解冻桑椹胚移植妊娠率与新鲜桑椹胚无显著差异(P>0.05);此外,移植桑椹胚时,移植侧有1个黄体的受体妊娠率极显著低于有2~3个黄体受体妊娠率(P<0.01),但移植囊胚时两者间差异不显著(P>0.05)。综上可知,子宫角移植桑椹胚和囊胚时,移植新鲜囊胚、移植冷冻桑椹胚效率较高;将新鲜桑椹胚移植给移植侧有2~3个黄体受体效果好于1个黄体受体。

关键词:绵羊;桑椹胚;囊胚;冷冻胚胎;受体黄体数;妊娠率

中图分类号:S826.3

文献标识码:A

DOI编号:10.19556/j.0258-7033.2017-07-048

Study of Fresh and Frozen Embryo Transfer Efficiency of Black Suffolk and Dorper Ewes

XU Zhi-yuan1,2, YANG Ming-hui2, JI Peng-yun2, ZHANG Xiao-sheng3, ZHANG Jin-long3, ZHU Shi-en2, Abulizi Wusiman4, ZHAO Jing1*, LIU Guo-shi2*

(1. College of Animal Science and Technology, Jilin Agricultural University, Jilin Changchun 130118, China; 2. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; 3. Tianjin Institute of Animal Sciences, Tianjin 300381, China; 4.College of Animal Science and Technology, Xinjiang Agricultural University, Xinjiang Urumqi 830052, China)

Abstract: To improve the embryo transfer efficiency of elite species of sheep with high meat quality, a total of 76 Black Suffolk and 27 Dorper ewes were treated by “CIDR+FSH+PMSG” method for superovulation as donors. The 384 smalltailed han sheep were treated by “CIDR+PMSG” method for estrus synchronization as receptors. It was explored to test the influence of developmental stages of embryo, frozen processing of the embryo, embryo in vitro residence time and transplantation of lateral corpus luteum number of receptor on the pregnancy rates after embryo transfer. The pregnancy rate of blastocyst transplantation was significantly higher than morula transplantation (P<0.01) when a single fresh embryo was transplanted. The frozen/thaw blastocyst transplantation pregnancy rate was significantly lower than fresh blastocyst (P<0.01), but the difference between frozen/thaw morula and fresh morula was not significant(P>0.05). In addition, the pregnancy rate with transplanted morula would be significantly affected (P<0.01), the transplanted blastocyst would not be significantly affected(P>0.05), when the transplanted-side-ovary of receptor had 1 corpus luteum or 2~3 corpus luteums. In conclusion, when morula and blastocyst were tansplanted into uterine horn, higher pregnancy rate could be achieved if a fresh blastocyst or a frozen morula was transfered. Fresh morula should be transplanted to the receptor with 2~3 corpus luteums on ovary transplantation side instead of 1 corpus luteum.

Key words: Sheep; Morula; Blastocyst; Frozen embryo; Corpus luteum number of receptor; Pregnancy rate

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